plasmid replicon meaning in English
质体复制子
Examples
- A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally
进一步序列分析表明该复制区至少有3个较大的orf ，分别编码501 ， 333 ， 183个氨基酸。其中orf1蛋白序列与hd263复制蛋白ori43的同源性为98 ，而另外两个orf和genbank己公布的bt复制相关蛋白无同源性。 30连续培养72h ，复制区质粒在bt无晶体突变株hd73cry ~ -中稳定性达98以上， 30h生长曲线也表明该复制区能够在bt中稳定复制和遗传，对受体菌株无明显不良影响。